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Human IL-40 (Interleukin 40) Microsample ELISA Kit

CAT: 0855-ELK0969MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK0969MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
IL-40; UNQ464
UniProt
Q6UX52
Reactivity
Human
Field of Research
Cytokine; Tumor immunity; Infection immunity; Cardiovascular biology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-40 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.13 ng/mL

UniProtKB · Q6UX52

Protein IL-40

IL40_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q6UX52
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C17orf99
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Adaptive immunityImmunity

Molecular function

Cytokine

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Glycoprotein

Cellular component

Secreted

Domain

Signal

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