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Mouse IL-27 (Interleukin 27) Microsample ELISA Kit

CAT: 0855-ELK9612MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK9612MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
IL-27; IL-27A; IL27p28; IL30; MGC71873; p28
UniProt
Q8K3I6
Reactivity
Mouse
Field of Research
Immunology; Cancer
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-27 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
19.12 pg/mL

UniProtKB · Q8K3I6

Interleukin-27 subunit alpha

IL27A_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q8K3I6
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Il27
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
IL-27 subunit alpha, IL-27-A, IL27-A
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

Molecular function

Cytokine

PTM

Glycoprotein

Biological process

ImmunityInflammatory responseInnate immunity

Cellular component

Secreted

Domain

Signal

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