EasyStep Human HP (Haptoglobin) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HP, and the Human HP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human HP. After TMB substrate solution is added, only those wells that contain Human HP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin; Hpt; Haptoglobin

• Reactivity:

  Human

• Field of Research:

  Infection immunity; Hematology

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HP, and the Human HP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Human HP. After TMB substrate solution is added, only those wells that contain Human HP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  1.5h

• Standard:

  400 ng/mL

• Sample Type:

  Serum, plasma

• Detection Range:

  6.25-400 ng/mL

• Sensitivity:

  0.02 ng/mL