EasyStep Human ALD (Aldosterone) ELISA Kit

• Product Name Alternative:

  ALD

• Reactivity:

  Human

• Field of Research:

  Metabolic pathway; Endocrinology; Hormone metabolism

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ALD antigen, and the Human ALD standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Human ALD to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human ALD in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  1.5h

• Standard:

  2000 pg/mL

• Sample Type:

  Extracted urine, serum, plasma

• Detection Range:

  31.25-2000 pg/mL

• Sensitivity:

  10.7 pg/mL