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Recombinant Mouse ApoE (C-6His)

CAT: 0855-EPT079Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0855-EPT079Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Apolipoprotein E (Apo-E), is a member of the apolipoprotein A1/A4/E family. ApoE is a major protein component of serum LDL, VLDL, HDL, and chylomicrons. APOE may function in mediating the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. APOE is usually secreted in plasma. Phosphorylation sites are present in the extracellular medium.
Description
Recombinant Mouse Apolipoprotein E is produced by our Mammalian expression system and the target gene encoding Glu19-Gln311 is expressed with a 6His tag at the C-terminus.
UniProt
P08226
Accession Number
P08226
Endotoxin
Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Molecular Weight
35 KDa
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Storage Temperature
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Notes
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Observed Molecular Weight
30-40 KDa, reducing conditions
Other Product Names
Apolipoprotein E; Apo-E; APOE

UniProtKB · P08226

Apolipoprotein E

APOE_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P08226
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Apoe
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
Apo-E
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Cellular component

ChylomicronEndosomeExtracellular matrixHDLSecretedVLDL

PTM

GlycoproteinOxidationPhosphoprotein

Molecular function

Heparin-binding

Biological process

Lipid transportTransport

Ligand

Lipid-binding

Domain

RepeatSignal