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Recombinant Mouse EGF (C-6His)

CAT: 0855-EPT032Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0855-EPT032Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
EGF is a single-pass type I membrane protein, containing 8 LDL-receptor class B repeats and 9 EGF-like domains. EGF results in cellular proliferation, differentiation, and survival.EGF is a low-molecular-weight polypeptide first purified from the mouse submandibular gland, but since then found in many human tissues including submandibular gland, parotid gland. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents.
Description
Recombinant Mouse Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn977-Arg1029 is expressed with a 6His tag at the C-terminus.
UniProt
P01132
Accession Number
P01132
Endotoxin
Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Molecular Weight
7.2 KDa
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Storage Temperature
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Notes
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Observed Molecular Weight
9-14 KDa, reducing conditions
Other Product Names
Pro-epidermal growth factor; Epidermal growth factor; EGF

UniProtKB · P01132

Pro-epidermal growth factor

EGF_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P01132
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Egf
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
EGF
EC number
—
Processing
Precursor
Secondary accessions
E9QNX6, Q569W5, Q6P9J2
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainRepeatSignalTransmembraneTransmembrane helix

Molecular function

Growth factor

Cellular component

Membrane