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Pig ACTb (Actin Beta) ELISA Kit

CAT: 0855-ELK8480-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK8480-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig ACTb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ACTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig ACTb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ACTb in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
UniProt
Q6QAQ1
Reactivity
Pig
Field of Research
Signal transduction; Developmental science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig ACTb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ACTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig ACTb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ACTb in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.56 ng/mL

UniProtKB · Q6QAQ1

Actin, cytoplasmic 1

ACTB_PIG · Sus scrofa

View on UniProt ↗
Primary accession
Q6QAQ1
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ACTB
Protein existence
1: Evidence at protein level
Organism
Sus scrofa (Pig)
Taxonomy ID
9823
Alternative names
—
EC number
3.6.4.-
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

PTM

AcetylationMethylationOxidationUbl conjugation

Ligand

ATP-bindingNucleotide-binding

Cellular component

CytoplasmCytoskeletonNucleus

Molecular function

Hydrolase

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