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E2 (Estradiol) ELISA Kit

CAT: 0855-ELK10219-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK10219-01Size:48 Tests
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Description
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with E2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
17B-Estradiol; Oestradiol; Beta-Estradiol
Reactivity
General
Field of Research
Endocrinology; Reproductive science; Hormone metabolism
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with E2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
3000 pg/mL
Sample Type
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Detection Range
46.88-3000 pg/mL
Sensitivity
14.45 pg/mL

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