Recombinant Human GDF-15 protein (His tag)

• Background:

  Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and-9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

• Abbreviation:

  GDF-15

• Synonyms:

  GDF-15; GDF15; Growth/differentiation factor 15

• UniProt:

  Q99988

• Accession Number:

  Q99988

• Expression System:

  E.coli

• Tag:

  C-His

• Sequence:

  Gln 151-Ile 308

• Field of Research:

  Cardiovascular; Cell Biology; signal Transduction

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  17.3 kDa

• Observed Molecular Weight:

  20 kDa

• Species:

  Human