Recombinant Human DnaJ

• Description:

  Recombinant Human DnaJ

• Product Name Alternative:

  Hsp40

• Gene Name:

  DNAJB1

• UniProt:

  P25685

• Cellular Locus:

  Cytoplasm , Nucleus , Nucleus, nucleolus , Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.

• Reactivity:

  Human

• Target Antigen:

  DnaJ homolog subfamily B member 1

• Target:

  DnaJ

• Type:

  Protein

• Applications:

  WB

• Concentration:

  Lot Specific

• Dilution:

  Dilute in PBS or medium that is identical to that used in the assay system.

• Form:

  Liquid

• Buffer:

  20 mM Tris

• Function:

  Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and rPubMed:24318877, PubMed:9499401}.

• Storage Conditions:

  Although stable at 4°C for 4 weeks, product should be stored at or below -20°C. For long term storage, addition of a carrier protein (0.1% HSA or BSA) is recommended.

• Specificity:

  DnaJ

• Formulation:

  Sterile-filtered colorless solution contains 20mM Tris buffer, pH 8.0, 2mM DTT, 0.2mM PMSF, and 10% glycerol.

• Buffer pH:

  pH 8.0

• Target Background:

  Human Hsp40 is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in protein folding, transport, and assembly. Hsp40 protein contains the J-domain which controls interactions with the ATPase domain of DnaK.

• CAS Number:

  9000-83-3