Anti-Hu SIGLEC10 APC

• Description:

  SIGLEC10 is a CD33-related receptor of sialoglycans, expressed on eosinophils, monocytes, a subpopulation of NK cells, and at lower level on B cells. Its murine ortholog is Siglec G. SIGLEC10 seems to act as an immunomodulatory receptor, which binds to VAP-1, a glycoprotein expressed on endothelium under inflammatory conditions. Another ligand of SIGLEC10 is CD24, a marker of poorer prognosis in carcinomas.

• Certification:

  RUO

• Reactivity:

  Human

• Immunogen:

  SIGLEC10 extracellular domain fused with human IgG1 Fc fragment

• Target Antigen:

  SIGLEC10

• Clone:

  5G6

• Applications:

  FC (QC tested)

• Format:

  APC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Stephenson HN, Mills DC, Jones H, Milioris E, Copland A, Dorrell N, Wren BW, Crocker PR, Escors D, Bajaj-Elliott M: Pseudaminic acid on Campylobacter jejuni flagella modulates dendritic cell IL-10 expression via Siglec-10 receptor: A novel flagellin-host interaction. J Infect Dis. 2014 Nov 1;210 (9) :1487-98., URL: https://pubmed.ncbi.nlm.nih.gov/24823621/, *Nguyen DH, Ball ED, Varki A: Myeloid precursors and acute myeloid leukemia cells express multiple CD33-related Siglecs. Exp Hematol. 2006 Jun;34 (6) :728-35., URL: https://pubmed.ncbi.nlm.nih.gov/16728277/, *Läubli H, Kawanishi K, Vazhappilly CG, Matar R, Merheb M, Siddiqui SS: Tools to study and target the Siglec-sialic acid axis in cancer. FEBS J. 2021 Nov;288 (21) :6206-6225., URL: https://pubmed.ncbi.nlm.nih.gov/33251699/, *Bhamidipati K, Silberstein JL, Chaichian Y, Baker MC, Lanz TV, Zia A, Rasheed YS, Cochran JR, Robinson WH: CD52 is elevated on B cells of SLE patients and regulates B cell function. Front Immunol. 2021 Feb 4;11:626820., URL: https://pubmed.ncbi.nlm.nih.gov/33658999/, *Munday J, Kerr S, Ni J, Cornish AL, Zhang JQ, Nicoll G, Floyd H, Mattei MG, Moore P, Liu D, Crocker PR: Identification, characterization and leucocyte expression of Siglec-10, a novel human sialic acid-binding receptor. Biochem J. 2001 Apr 15;355 (Pt 2) :489-97., URL: https://pubmed.ncbi.nlm.nih.gov/11284738/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 5G6 recognizes an extracellular epitope of human SIGLEC10, a sialic acid-binding lectin expressed on subsets of human leucocytes.

• Applications Notes:

  6

• Isotype:

  Mouse IgG1