Human IFN-γ (Interferon Gamma) ELISPOT Kit

• Abbreviation:

  IFN-γ

• UniProt:

  P01579

• Cross Reactivity:

  This kit recognizes Human IFN-γ in samples.No significant cross-reactivity or interference between Human IFN-γ and analogues was observed.

• Target:

  IFNG; IFG; IFI; Type II Interferon

• Applications:

  ELISPOT

• Field of Research:

  Cell Biology; Immunology; Neuroscience

• Assay Principle:

  This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IFN-γ antibodies. During the experiment, cell suspension and stimulants are added first, and IFN-γ secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IFN-γ antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IFN-γ antibodies will bind specifically to the IFN-γ which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IFN-γ secreting cells.

• Assay Performance Time:

  3 h 30 min

• Sample Type:

  Suspension cell

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,12 months

• Species:

  Human

• Recommended Usage:

  This ELISPOT kit is designed for the detection of the frequency of human IFN-γ secreting cells.