Human IFN-β (Interferon Beta) ELISA Kit

IFN-β

P01574

This kit recognizes Human IFN-β in samples. No significant cross-reactivity or interference between Human IFN-β and analogues was observed

IFNB1; IFB; IFF; IFNB

Sandwich-ELISA

ELISA

Cancer; Cardiovascular; Immunology

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFN-β. You can calculate the concentration of Human IFN-β in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Serum, plasma and other biological fluids

31.25-2000 pg/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

18.75 pg/mL

Ice bag

2-8°C,12 months

https://www.nature.com/articles/s41392-021-00703-3

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120224/

https://www.sciencedirect.com/science/article/pii/S1521661623000177

https://www.nature.com/articles/s41388-021-01869-4

https://www.nature.com/articles/s41419-020-2665-z

https://www.sciencedirect.com/science/article/pii/S2211124719300397

https://link.springer.com/article/10.1186/s13578-021-00687-1

https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1009366

https://www.nature.com/articles/s41419-020-2625-7

https://www.frontiersin.org/articles/10.3389/fimmu.2017.01051/full

https://www.frontiersin.org/articles/10.3389/fimmu.2017.00779/full

https://www.mdpi.com/1090868

Microplate reader

Human