BRK Kinase Assay Kit

CAT: 0421-78841Size: 96 ReactionsDry Ice: YesHazardous: No

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CAT#:0421-78841Size:96 Reactions

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Background

Breast Tumor Kinase (BRK, also known as Tyrosine-protein kinase-6 or PKT6) is a non-receptor protein with tyrosine kinase activity. BRK is involved in epithelial differentiation and apoptosis. It interacts with multiple proteins and is involved in several signaling pathways, like activating STAT3 and STAT5B to promote cell proliferation. It is involved in cancer progression, migration and evasion, namely in breast cancer. Targeting BRK kinase activity via small molecule inhibitors may be a viable therapeutic approach for breast cancer.

Description

The breast tumor kinase (BRK) Kinase Assay Kit is designed to measure BRK tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent.

UniProt

Q13882

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage40403BRK, GST-Tag*10 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°C40217PTK Substrate (Poly-Glu, Tyr 4:1) (10 mg/ml) 50 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For further questions, please email[email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

UniProtKB · Q13882

Protein-tyrosine kinase 6

PTK6_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q13882
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: PTK6
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: 2.7.10.2
Processing: —
Secondary accessions: B2RCR3, B4DW46, Q58F01

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell projectionCytoplasmMembraneNucleus

Molecular function

KinaseTransferaseTyrosine-protein kinase

PTM

Phosphoprotein

Domain

SH2 domainSH3 domain

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