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Wee1 Kinase Assay Kit

CAT: 0421-79909Size: 96 ReactionsDry Ice: YesHazardous: No
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CAT#:0421-79909Size:96 Reactions
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Wee1 (also known as WEE1 G2 checkpoint kinase) belongs to the serine-threonine kinase family of proteins. Wee1 plays a crucial role in regulating G2/M transition through phosphorylation of cyclin dependent kinase (CDKs), allowing DNA damage to be repaired. that is overexpressed in many types of cancer such as luminal and HER2-positive breast cancer, hepatocellular carcinomas, and glioblastomas. Classical cancer therapies, such as chemotherapy and radiotherapy, induce DNA damage, and cells trigger the checkpoint in cell cycle, such as Wee1. This can lead to cancer cell resistance. Inhibiting Wee1 prevents cells from repairing DNA damage due to unchecked replication, suggesting that combination therapeutics with DNA damaging reagents and Wee1 inhibitors may be effective against cancer.
Description
The Wee1 Kinase Assay Kit is designed to measure Wee1 kinase activity for screening and profiling applications using Kinase-Glo® Kinase Assay as a detection reagent. The Wee1 Kinase Assay Kitcomes in a convenient 96-well format, with enough purified recombinant Wee1 enzyme (amino acids 215-646), Wee1 Substrate, ATP, and kinase assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against Wee1? Check out ourKinase Screening ServicesorDNA Replication and Repair Screening Services.
UniProt
P30291
Applications
Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Format
Catalog #NameAmountStorage40412Wee1, GST-Th-Tag*15 μg-80°C793345x Kinase Assay Buffer 11.5 ml-20°C79686500 µM ATP100 µl-20°C101574Wee1 Substrate (5 mg/ml) 200 µl-80°C79696White 96-well plate1RoomTemp.*The concentration of the protein is lot-specific and will be indicated on the tube
Shipping Conditions
-80°C
Storage Conditions
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Contraindications
The final concentration of DMSO in the assay should not exceed 1%.

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Alternative Products

CatalogName
MBS6004282-01Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase)
MBS6363511-01Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (AP)
MBS6363513-01Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (Biotin)
MBS6363521-01Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (PE)
MBS6363512-01Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (APC)
MBS6363514-01Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (FITC)
759331BKWEE1 Binding Assay Kit
043866-APWee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (AP) discontinued
043866-PEWee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (PE) discontinued
MBS6363517-01Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (MaxLight 490)

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