Wee1 Kinase Assay Kit

CAT: 0421-79909Size: 96 ReactionsDry Ice: YesHazardous: No

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CAT#:0421-79909Size:96 Reactions

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Background

Wee1 (also known as WEE1 G2 checkpoint kinase) belongs to the serine-threonine kinase family of proteins. Wee1 plays a crucial role in regulating G2/M transition through phosphorylation of cyclin dependent kinase (CDKs), allowing DNA damage to be repaired. that is overexpressed in many types of cancer such as luminal and HER2-positive breast cancer, hepatocellular carcinomas, and glioblastomas. Classical cancer therapies, such as chemotherapy and radiotherapy, induce DNA damage, and cells trigger the checkpoint in cell cycle, such as Wee1. This can lead to cancer cell resistance. Inhibiting Wee1 prevents cells from repairing DNA damage due to unchecked replication, suggesting that combination therapeutics with DNA damaging reagents and Wee1 inhibitors may be effective against cancer.

Description

The Wee1 Kinase Assay Kit is designed to measure Wee1 kinase activity for screening and profiling applications using Kinase-Glo® Kinase Assay as a detection reagent. The Wee1 Kinase Assay Kitcomes in a convenient 96-well format, with enough purified recombinant Wee1 enzyme (amino acids 215-646), Wee1 Substrate, ATP, and kinase assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against Wee1? Check out ourKinase Screening ServicesorDNA Replication and Repair Screening Services.

UniProt

P30291

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage40412Wee1, GST-Th-Tag*15 μg-80°C793345x Kinase Assay Buffer 11.5 ml-20°C79686500 µM ATP100 µl-20°C101574Wee1 Substrate (5 mg/ml) 200 µl-80°C79696White 96-well plate1RoomTemp.*The concentration of the protein is lot-specific and will be indicated on the tube

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

UniProtKB · P30291

Wee1-like protein kinase

WEE1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P30291
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: WEE1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: WEE1hu
EC number: 2.7.10.2
Processing: —
Secondary accessions: B3KVE1, D3DQV0

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Biological process

Cell cycleCell divisionMitosis

Molecular function

KinaseTransferaseTyrosine-protein kinase

Cellular component

Nucleus

PTM

PhosphoproteinUbl conjugation

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Alternative Products

Catalog	Name
MBS6004282-01	Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase)
MBS6363511-01	Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (AP)
MBS6363513-01	Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (Biotin)
MBS6363521-01	Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (PE)
MBS6363512-01	Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (APC)
MBS6363514-01	Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (FITC)
759331BK	WEE1 Binding Assay Kit
043866-AP	Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (AP) discontinued
043866-PE	Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (PE) discontinued
MBS6363517-01	Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (MaxLight 490)