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TET2 Homogeneous Assay Kit

CAT: 0421-78559Size: 384 ReactionsDry Ice: YesHazardous: No
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CAT#:0421-78559Size:384 Reactions
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Background
TET2 (also known as Tet Methylcytosine dioxygenase 2) belongs to the Ten Eleven Translocation (TET) family of proteins that catalyze 5-methylcytosine oxidation and generate 5-methylcytosine derivatives, including 5-hydroxymethylcytosine. TET2 regulates gene expression levels in cancer and immune cells, being a widely recognized tumor suppressor gene. Both homozygous and heterozygous mutations in TET2 are indicative of hematopoietic malignancies, with nonsense and frameshift mutations associated with poor prognosis. In addition, TET2 is one of the most frequently mutated genes in acute myeloid leukemia (AML) . Targeting TET2 as therapeutical approach may be complex, as in many cancer types TET2 is inactivated, however it can still be found in some immune cell types and it can be modulated via proteins that regulate TET2 activity.
Description
The TET2 Homogeneous Assay Kitis designed to measure the activity of TET2 (Tet Methylcytosine dioxygenase 2) for screening and profiling applications. TheTET2 Homogeneous Assay Kitcomes in a convenient AlphaLISA® format, with enough biotinylated TET2 substrate, TET2 assay buffer, and purified recombinant human TET2 (amino acids 1129-2002 (end) ) for 384 reactions.Figure 1: TET2 Homogeneous Assay Kit schematic.First, a sample containing TET2 enzyme is incubated with the reaction mixture. Next, acceptor beads are added, followed by donor beads and reading the Alpha-counts. The increase in Alpha-counts is proportional to the hydroxymethylation of the substrate. C = cytosine; HC = hydroxylated cytosine.Need us to run inhibitor screens or profile your compounds against TET2? Check out ourDemethylase Screening Services.
UniProt
Q6N021
Applications
Screen for molecules that inhibit TET2 activity.
Format
Catalog #NameAmountStorage50162TET2, FLAG-Tag, His-Tag (catalytic domain) 2 x 40 µg-80°C82853Biotin-labeled TET2 Substrate (4 µM) 2 x 25 µl-80°C82854Primary Antibody 27-24 µl-80°C798324x TET2 Assay Buffer 13 ml-80°C523014x Detection Buffer 12 x 2 ml-20°CPlate sealer1Room Temp
Shipping Conditions
-80°C
Storage Conditions
This assay kit will perform optimally for up to6 monthsfrom date of receipt when the materials are stored as directed.
Notes
Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]
Contraindications
The TET2 Homogenous Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 4% DMSO solution in buffer and using 2.5 µl per well.Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue, interfere with the assay.Avoid using potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+and Ni2+) .The presence of the culture medium RPMI 1640 at > 1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.

UniProtKB · Q6N021

Methylcytosine dioxygenase TET2

TET2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q6N021
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TET2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
1.14.11.80
Processing
—
Secondary accessions
B5MDU0, Q2TB88, Q3LIB8, Q96JX5, Q9HCM6, Q9NXW0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationGlycoproteinIsopeptide bondMethylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

Cell cycle

Molecular function

Chromatin regulatorDioxygenaseDNA-bindingOxidoreductase

Cellular component

ChromosomeNucleus

Disease

Disease variantTumor suppressor

Ligand

IronMetal-bindingZinc

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