5-Hydroxymethylcytosine (5-hmC) Recombinant Mouse Monoclonal Antibody [PSH06-08]

5-Hydroxymethylcytosine (5-hmC) Recombinant Mouse Monoclonal Antibody [PSH06-08]

• Description: 5-Hydroxymethylcytosine (5hmC) is a DNA pyrimidine nitrogen base derived from cytosine. It is potentially important in epigenetics, because the hydroxymethyl group on the cytosine can possibly switch a gene on and off. It was first seen in bacteriophages in 1952. However, in 2009 it was found to be abundant in human and mouse brains, as well as in embryonic stem cells. In mammals, it can be generated by oxidation of 5-methylcytosine, a reaction mediated by TET enzymes.
• Product Name Alternative: 5-hmC antibody 5-hydroxymethylcytosine antibody 5hmC antibody
• Abbreviation: 5hmC
• CAS Number: 9000-83-3
• Host: Mouse
• Species Reactivity: Universal
• Immunogen: Recombinant protein fused with 5-Hydroxymethylcytosine.
• Isotype: IgG1
• Clone: PSH06-08
• Conjugation: Non-conjugated
• Type: Recombinant Mouse monoclonal Antibody
• Applications: WB, IHC-P, IF-Cell, Dot Blot, ELISA, MeDIP
• Positive Control: Human colon tissue, human colon cancer tissue, HeLa.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IHC-P: 1:200 ;IF-Cell: 1:100 ;Dot Blot: 1:1,000 ;MeDIP: Use 5 μg for 3 μg of chromatin.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes