Products for Research Use Only
RegisterLogin

ABTS 1-Component HRP Microwell Substrate (SUBA)

CAT: 0013-GTR19350600Size: 100 mLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR19350600Size:100 mL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
ABTS 1-Component HRP Microwell Substrate is a ready-to-use solution containing 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) in a mildly acidic buffer. This substrate is ideal for use in ELISAs where the detection level is in the μg-ng/mL range and horseradish peroxidase (HRP) is the conjugated detection enzyme. ABTS 1-Component HRP Microwell Substrate is suitable for ELISAs where the test samples contain high concentrations of the target molecule or when it is advantageous to utilize lower sample dilution factors.
Concentration
1 X
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
1. Run ELISA according to the specific protocol through the conjugate incubation step. 2. Wash the wells three or four times with 1X ELISA Wash Buffer to remove any residual, AP-conjugate. 3. Bring SUBP to room temperature; protect from light. 4. Pipette 100 μL SUBP into each well of the plate. 5. Incubate SUBP 10-60 minutes. Monitor the color intensity. 6. Read the plate between 405-420 nm and analyze. Alternatively, stop the reaction by adding 100 μL/well STOPP and read the samples between 405-420 nm within 1 hour.

Related Products

CatalogName
GTR19286728TMB 1-Component HRP Microwell Substrate (SUBT)
GTR19286727TMB 1-Component HRP Microwell Substrate (SUBT)
GTR19286698TMB Slow Kinetic 1-Component HRP Microwell Substrate (SUBK)
GTR19286697TMB Super Sensitive 1-Component HRP Microwell Substrate (SUBS)
GTR19286696TMB Super Sensitive 1-Component HRP Microwell Substrate (SUBS)
GTR19300569PNPP 1-Component AP Microwell Substrate with Stabilizing Pellets (SUBP)
GTR19286729TMB 1-Component HRP Membrane Substrate (SUBM)
GTR19286704TMB One Component Substrate
GTR19346758BCIP/NBT 1-Component AP Membrane Substrate (SUBB)
GTR19286711TMB HRP Substrate

Alternative Products

CatalogName
MBS258417-01ABTS 1-Component HRP Microwell Substrate (SUBA)
MBS258075-01pNPP 1-Component AP Microwell Substrate
MBS258392-01TMB 1-Component HRP Microwell Substrate
6276TMB 1-Component HRP Microwell Substrate (SUBT)
MBS258391-01TMB Super Sensitive 1-Component HRP Microwell Substrate
MBS258074-01TMB Slow Kinetic 1-Component HRP Microwell Substrate
6275TMB Super Sensitive 1-Component HRP Microwell Substrate (SUBS)
6279pNPP 1-Component AP MicroWell Substrate with Stabilizing Pellets (SUBP)
6277TMB Slow Kinetic 1-Component HRP Microwell Substrate (SUBK)
172023-01ImmunoTek PlusTM Microwell Peroxidase Substrate, Single Component discontinued

Popular Products