Hydroxyphenyl Fluorescein

• UNSPSC Description:

  Hydroxyphenyl Fluorescein (HPF) is a stable ROS fluorescent probe dye. Hydroxyphenyl Fluorescein has stronger specificity and stability than H2DCFDA (HY-D0940). Hydroxyphenyl Fluorescein can produce strong green fluorescence through hydroxyl radical reaction with intracellular peroxynitroso. Hydroxyphenyl Fluorescein can be applied for fluorescence microscopy, high-throughput imager, luciferase microplate reader or flow cytometry. Ex/Em=490/515 nm[1].

• Target Antigen:

  Fluorescent Dye; Reactive Oxygen Species

• Type:

  Dye Reagents

• Related Pathways:

  Immunology/Inflammation;Metabolic Enzyme/Protease;NF-κB;Others

• Applications:

  Metabolism-sugar/lipid metabolism

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/hydroxyphenyl-fluorescein.html

• Purity:

  99.91

• Solubility:

  DMSO : 125 mg/mL (ultrasonic)

• Smiles:

  OC1=CC=C(OC2=CC3=C(C4(C5=CC=C(C=C5O3)O)C6=C(C(O4)=O)C=CC=C6)C=C2)C=C1

• Molecular Weight:

  424.40

• References & Citations:

  [1]Ming-De Li, et al. Dynamics of Oxygen-Independent Photocleavage of Blebbistatin as a One-Photon Blue or Two-Photon Near-Infrared Light-Gated Hydroxyl Radical Photocage. J Am Chem Soc. 2018 Nov 21;140(46):15957-15968.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  -20°C (Powder, protect from light)

• Clinical Information:

  No Development Reported

• CAS Number:

  359010-69-8