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Fluorescein tyramide

CAT: 0804-HY-131008-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0804-HY-131008-01Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fluorescein tyramide is a green fluorescent reagent (λabs: 494 nm; λem: 517 nm) . Fluorescein tyramide is widely used for tyramide signal amplification (TSA) with a low-abundance in IHC, ICC, in situ hybridization (FISH) and flow cytometry (FCM) applications[1][2].
CAS Number
210236-90-1
UNSPSC
12171500
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/fluorescein-tyramide.html
Purity
99.0
Solubility
10 mM in DMSO
Smiles
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=CC=C5.O=C(C)NCCC6=CC=C(O)C=C6
Molecular Formula
C29H21NO7
Molecular Weight
495.49
References & Citations
[1]A H Hopman, et al. Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification. J Histochem Cytochem. 1998 Jun;46 (6) :771-7.|[2]R P van Gijlswijk, et al. Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J Histochem Cytochem. 1997 Mar;45 (3) :375-82.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C, 3 years (Powder)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

Chemical Information

3',6'-Dihydroxy-N-[2-(4-hydroxyphenyl)ethyl]-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxamide
CAS Number210236-90-1
PubChem CID162640808
IUPAC Name3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one;N-[2-(4-hydroxyphenyl)ethyl]acetamide
Molecular FormulaC30H25NO7
Molecular Weight511.5
Topological Polar Surface Area125
Hydrogen Bond Donor Count4
Hydrogen Bond Acceptor Count7
Rotatable Bond Count3
Heavy Atom Count38
Formal Charge0
Complexity685
SMILES
CC(=O)NCCC1=CC=C(C=C1)O.C1=CC=C2C(=C1)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
InChI
InChI=1S/C20H12O5.C10H13NO2/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;1-8(12)11-7-6-9-2-4-10(13)5-3-9/h1-10,21-22H;2-5,13H,6-7H2,1H3,(H,11,12)
InChIKey
RIHCPYWHAYBEHO-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of 3',6'-Dihydroxy-N-[2-(4-hydroxyphenyl)ethyl]-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxamide
CAS: 210236-90-1
CID: 162640808
Formula: C30H25NO7
MW: 511.5
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight511.5
Hydrogen Bond Donor Count4
Hydrogen Bond Acceptor Count7
Rotatable Bond Count3
Exact Mass511.16310214
Monoisotopic Mass511.16310214
Topological Polar Surface Area125 Ų
Heavy Atom Count38
Formal Charge0
Complexity685
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count2
Compound Is CanonicalizedYes

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