H2DCFDA

• Description:

  H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm) [1].

• Product Name Alternative:

  DCFH-DA; 2',7'-Dichlorodihydrofluorescein diacetate

• UNSPSC:

  12171500

• Hazard Statement:

  H302, H315, H319, H335

• Target:

  Reactive Oxygen Species (ROS)

• Type:

  Dye Reagents

• Related Pathways:

  Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/H2DCFDA.html

• Purity:

  99.82

• Solubility:

  DMSO : 250 mg/mL (ultrasonic)

• Smiles:

  O=C(O)C1=CC=CC=C1C2C3=C(OC4=C2C=C(Cl)C(OC(C)=O)=C4)C=C(OC(C)=O)C(Cl)=C3

• Molecular Formula:

  C24H16Cl2O7

• Molecular Weight:

  487.29

• Precautions:

  H302, H315, H319, H335

• References & Citations:

  [1]Lyublinskaya OG, et al. Redox environment in stem and differentiated cells: A quantitative approach. Redox Biol. 2017 Aug;12:758-769.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  -20°C (Powder, protect from light)

• Scientific Category:

  Dye Reagents

• Clinical Information:

  No Development Reported

• CAS Number:

  [4091-99-0]