Recombinant Human CD40

• Product Name Alternative:

  B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

• UniProt:

  P25942

• Expression Region:

  21-193

• Host:

  CHO cells

• Tag:

  C-terminal His Tag

• Applications:

  Western Blot, ELISA

• Field of Research:

  Immunology, Signal Transduction

• Endotoxin:

  < 1 EU/µg as determined by LAL test.

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Measured by its binding ability in a functional ELISA.Immobilized recombinant human CD40 at 1 μg/mL. (100 μl/well) can bind biotinylated human CD40L with a linear range of 25-600 ng/mL.

• Form:

  Lyophilized powder

• Reconstitution:

  Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

• Molecular Weight:

  32 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

• Formulation:

  Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.

• Organism Species:

  Human