FITC Anti-Human CD40 Antibody (3A8)

• Background:

  CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand) . The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.

• Synonyms:

  B-cell surface antigen CD40|Bp50|CD40|CD40L receptor|Cd40|Tnfrsf5|Tumor necrosis factor receptor superfamily member 5

• Gene ID:

  958

• UniProt:

  P25942

• Host:

  Mouse

• Reactivity:

  Human

• Target:

  Human CD40

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Conjugation:

  FITC

• Dilution:

  Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood) . Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

• Form:

  Liquid

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Clone ID:

  3A8

• Tested Applications:

  FC

• Conjugation Information:

  FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter) .