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RNase Inhibitor - recombinant

CAT: 0013-GTR19296890Size: 2000 UnitsDry Ice: NoHazardous: No
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CAT#:0013-GTR19296890Size:2000 Units
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RNase inhibitor is a recombinant protein which completely inhibits a broad spectrum of eukaryotic RNases, including RNase A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity (4 x 10-14M) . It does not inhibit the RNases I, T1, T2, H, U1, U2 and Cl3. In addition, RNase inhibitor shows no inhibition of polymerase or reverse transcriptase activity and so can be used for cDNA synthesis and in one-step RT-PCR reactions. The murine version of RNase inhibitor lacks the pair of cysteines identified in human version, therefore it has significantly improved resistance to oxidation.
Product Name Alternative
Mus musculus, recombinant
Field of Research
Pharmacology & Drug Discovery
Concentration
40 units/μl
Form
Liquid
Storage Conditions
Store at -20 °C. avoid freeze/thaw cycles
Notes
For research use only.
Applications Notes
RNase inhibitor should be applied in a working concentration of 40 units (1 μl) per 50 μl reaction volume. The application of RNase in-hibitor is recommended for: cDNA synthesis/RT-PCR; In vitro transcription/translation; RNA purification; RNA protection assays; Separation and identification of specific ribonuclease activities; Other applications where the integrity of RNA is essentia.

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