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Fluorescein-12-dCTP

CAT: 0013-GTR19207261Size: 10 ulDry Ice: NoHazardous: No
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CAT#:0013-GTR19207261Size:10 ul
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fluorescein-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Fluorescein-12-dCTP/dCTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dCTP-/70-50% dCTP.
Product Name Alternative
Fluorescein-X-5-Propargylamino-dCTP, 5/6-Fluorescein-X-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Solution in 10 mM Tris-HCl; Color: yellow-orange; pH: 7.5 ± 0.5
Smiles
[C@@H]1 (C[C@@H] (O[C@@H]1COP (=O) (OP (=O) (OP (=O) (O) O) O) O) n1c (=O) nc (c (c1) C#CCNC (=O) CCCCCNC (=O) c1ccc (c (c1) c1c2c (oc3c1ccc (=O) c3) cc (cc2) O) C (=O) O) N) O.[C@@H]1 (C[C@@H] (O[C@@H]1COP (=O) (OP (=O) (OP (=O) (O) O) O) O) n1c (=O) nc (c (c1) C#CCNC (=O) CCCCCNC (=O) c1ccc (c (c1) C (=O) O) c1c2c (oc3c1ccc (=O) c3) cc (cc2) O) N) O
Molecular Formula
C39H40N5O20P3
Molecular Weight
Theoretical MW: 991.68 g/mol; Detected MW: 991.15 g/mol
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 492 nm, λem 517 nm, ε 83.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) .

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Alternative Products

CatalogName
NU-809-FAMX-LFluorescein-12-dCTP
JBS-NU-809-FAMX-LFluorescein-12-dCTP, L pack
JBS-NU-809-FAMX-SFluorescein-12-dCTP, S pack
NU-809-RHOX-SRhodamine-12-dCTP
17035-AATFluorescein-12-dCTP *1 mM in Tris Buffer (pH 7.5) *
B8207-.025Fluorescein-12-dUTP
B8332-.025Fluorescein-12-UTP
NU-1611-FAMX-LFluorescein-12-dATP
NU-1619-FAMXFluorescein-12-ddUTP
JBS-NU-1612-FAMXFluorescein-12-ddATP

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