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Rhodamine-12-dCTP

CAT: 0013-GTR19207259Size: 10 ulDry Ice: NoHazardous: No
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CAT#:0013-GTR19207259Size:10 ul
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Rhodamine-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Rhodamine-12-dCTP/dCTP ratio for Nick Trans-lation: 30-50% Rhodamine-12-dCTP/70-50% dCTP.
Product Name Alternative
Rhodamine-X-5-propargylamino-dCTP, Rho-green-X-dCTP, RhG-dCTP, R110-dCTP
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Solution in water; Color: orange; pH: 7.5 ± 0.5
Smiles
N (CC#Cc1cn (c (=O) nc1N) [C@@H]1O[C@H] (COP (=O) (O) OP (=O) (O) OP (=O) (O) O) [C@@H] (O) C1) C (=O) CCCCCNC (=O) c1ccc (c (c1) c1c2ccc (cc2oc2-c1ccc (=[NH2+]) c2) N) C (=O) [O-]
Molecular Formula
C39H42N7O18P3
Molecular Weight
Theoretical MW: 989.71 g/mol (free acid) ; Detected MW: 989.18 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Incorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 505 nm, λem 530 nm, ε 85.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) .

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Alternative Products

CatalogName
NU-809-RHOX-SRhodamine-12-dCTP
JBS-NU-809-RHOX-SRhodamine-12-dCTP, S pack
JBS-NU-809-RHOX-LRhodamine-12-dCTP, L pack
NU-809-FAMX-lFluorescein-12-dCTP
NU-803-RHOX-lRhodamine-12-dUTP
JBS-NU-809-FAMX-LFluorescein-12-dCTP, L pack
JBS-NU-809-FAMX-SFluorescein-12-dCTP, S pack
JBS-NU-803-RHOX-SRhodamine-12-dUTP, S pack
JBS-NU-803-RHOX-LRhodamine-12-dUTP, L pack
296054DCTP

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