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Fluorescein-12-dUTP

CAT: 0013-GTR19207291Size: 10 ulDry Ice: NoHazardous: No
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CAT#:0013-GTR19207291Size:10 ul
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fluorescein-12-dUTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Fluorescein-12-dUTP/dTTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dUTP/70-50% dTTP.
Product Name Alternative
Fluorescein-X- (5-aminoallyl) -dUTP, 5/6-Fluorescein-X- (5-aminoallyl) -2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Solution in water; Color: yellow-orange; pH: 7.5 ± 0.5
Smiles
O=c1ccc2c (c1) oc1cc (O) ccc1c2c1c (ccc (c1) C (=O) NCCCCCC (=O) NC/C=C/c1cn (c (=O) [nH]c1=O) [C@@H]1O[C@H] (COP (=O) (O) OP (=O) (O) OP (=O) (O) O) [C@@H] (O) C1) C (=O) O.O=c1c (cn (c (=O) [nH]1) [C@@H]1O[C@H] (COP (=O) (O) OP (=O) (O) OP (=O) (O) O) [C@@H] (O) C1) /C=C/CNC (=O) CCCCCNC (=O) c1cc (c (c2c3ccc (O) cc3oc3cc (=O) ccc23) cc1) C (=O) O
Molecular Formula
C39H41N4O21P3
Molecular Weight
Theoretical MW: 994.69 g/mol (free acid) ; Detected MW: 994.15 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 492 nm, λem 517 nm, ε 83.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) .

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