Sheep anti Mouse 3BP2 (SH3 Binding Protein 2)

• Background:

  3BP2 (SH3 binding protein 2) was identified as a protein binding to the SH3 domain of the Abl proto-oncogene. Murine 3BP2 cDNA encodes a 559 amino acid protein of 85 kDa consisting of an N-terminal PH domain, a C-terminal SH2 domain and intervening proline-rich sequences. 3BP2 interacts via its Sh2 domain with Syk family protein tyrosine kinases Syk, Zap70, Grb2, Cbl, PLC 1 and LAT in T lymphocytes and contributes to the transcriptional activation of NF-AT and AP-1. Mouse 3BP2 is 66% homologous to the Human protein at the amino acid level.

• Synonyms:

  Sh3bp2, SH3 Domain-Binding Protein 2

• UniProt:

  Q06649

• Host:

  Sheep

• Species Reactivity:

  Mouse

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  WB

• Field of Research:

  Signal Transduction

• Purification Method:

  Ammonium Sulfate Precipitation

• Assay Principle:

  Detects 3BP2 protein by Western blot analysis. Detects a 85 kDa band in murine fibroblasts transfected with 3BP2. Optimal concentration should be evaluated by serial dilutions.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Decker, M., et al. Adaptor function for the Syk kinases-interacting protein 3BP2 in IL-2 gene activation. Immunity 1998, 9, 595-605 2. Songyang, Z., et al. Specific motifs recognized by the SH2 domains of Csk, 3BP2, fps/fes, GRB-2, HCP, SHC, Syk and Vav. Mol. Cell Biol. 1994, 14, 2777-2785

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4