Sheep anti Mouse LFC
CAT:
579-X1089P
Size:
250 µg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Sheep anti Mouse LFC
- Background: Lfc is a 118 kDa protein discovered in a screen for oncogenes in NIH 3T3 cells. Lfc is a signaling protein related to Lbc and is part of the Dbl family of proteins. Lfc is a guanine-nucleotide exchange factor (GEF) for the Rho family of small GTPases. Lfc has also been shown to be upstream of the serine-threonine kinase JNK. Lfc has also been shown to mediate the activation of the Rac signaling pathway.
- Synonyms: Rho guanine nucleotide exchange factor 2, ARHGEF2
- CAS Number: 9007-83-4
- UniProt: Q92974 (Human)
- Host: Sheep
- Species Reactivity: Mouse
- Isotype: IgG
- Conjugation: Unconjugated
- Type: Polyclonal Antibody
- Applications: WB, IP, IF
- Field of Research: Signal Transduction
- Purification Method: Ammonium Sulfate Precipitation
- Assay Principle: Detects Lfc in mouse tissues by immunoprecipitation-Western blot at 4 µg/mL. Detects a 118 kDa band in mouse tissue extracts corresponding to wild-type Lfc. Can also be used in immunofluorescence assays. Optimal concentration should be evaluated by serial dilutions.
- Stability: See expiration date on vial
- Concentration: See vial for Concentration
- Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
- Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
- References & Citations: 1. Glaven, J.A., et al. The Dbl-related protein, Lfc, localizes to microtubules and mediates the activation of Rac signaling pathways in cells. J. Biol. Chem. 1999, 274, 2279-2285 2. Glaven, J.A., et al. Lfc and Lsc oncoproteins represent two new guanine nucleotide exchange factors for the Rho GTP-binding protein. J. Biol. Chem. 1996, 271, 27374-27381 3. Whitehead, I., et al. Expression cloning of lfc, a novel oncogene with structural similarities to guanine nucleotide exchange factors and to the regulatory region of protein kinase C. J. Biol. Chem. 1995, 270, 18388-18395
- Shipping Conditions: Ambient Temperature, freeze upon arrival
- Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
- Functional Analysis: WB