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Recombinant Human CD40L Protein

CAT: 0013-GTR19125282-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19125282-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Human CD40 Ligand is produced by our E.coli expression system and the target gene encoding Met113-Leu261 is expressed.
Product Name Alternative
CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP
UniProt
P29965
Expression Region
Met113-Leu261
Tag
Tag free
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
<1EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
ELISA: Immobilized Recombinant Human CD40 (C-Fc) (Cat#orb2994010) at 2μg/ml (100 μl/well) can bind Recombinant Human CD40L (Cat#orb2995248) *.*: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human CD40L (Cat#orb2995248) is 1-10 ng/ml. (QC verified) . BLI: Loaded Human CD40-His (Cat#orb2995146) on HIS1K Biosensor, can bind Human CD40L (Cat#orb2995248) with an affinity constant of 2.43 nM as determined in BLI assay. (Regularly tested) Loaded Anti-Human CD40L mAb-Fc (Cat#orb2993151) on Protein A Biosensor, can bind Human CD40L (Cat#orb2995248) with an affinity constant of 0.05 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 16.2 kDa. Observed: 15 kDa, reducing conditions
References & Citations
Huey-Liang Kuo, Chiu-Ching Huang, Tze-Yi Lin, Ching-Yuang Lin IL-17 and CD40 ligand synergistically stimulate the chronicity of diabetic nephropathy Nephrology Dialysis Transplantation, 33, 248 (2018)
Pubmed id
28339909
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.

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