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Recombinant Macaca mulatta Leukocyte surface antigen CD47 (CD47), partial (Active)

CAT: 0013-GTR19123494-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19123494-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This Recombinant Macaca mulatta Leukocyte surface antigen CD47 (CD47), partial (Active) spans the amino acid sequence from region 19-141aa. Purity: Greater than 95% as determined by SDS-PAGE.
UniProt
F7A802
Expression Region
19-141aa
Tag
C-terminal 10xHis-tagged
Source
Mammalian cell
Origin
Macaca mulatta (Rhesus macaque)
Field of Research
Immunology & Inflammation; Cell Biology
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
Greater than 95% as determined by SDS-PAGE.
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta CD47 at 2 μg/mL can bind Anti-CD47 recombinant antibody. The EC50 is 4.473-4.933 ng/mL.
Form
Lyophilized powder
Molecular Weight
15.3 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
AA Sequence
QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTAPANFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNE

UniProtKB · F7A802

Leukocyte surface antigen CD47

F7A802_MACMU · Macaca mulatta

View on UniProt ↗
Primary accession
F7A802
Review status
UniProtKB unreviewed (TrEMBL)
Gene
CD47
Protein existence
4: Predicted
Organism
Macaca mulatta (Rhesus macaque)
Taxonomy ID
9544
Alternative names
—
EC number
—
Processing
—
Secondary accessions
F7F5Z4
Protein keywords

Biological process

Cell adhesion

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinPhosphoproteinPyrrolidone carboxylic acid

Domain

Immunoglobulin domainSignalTransmembraneTransmembrane helix

Technical term

Reference proteome

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