Rabbit anti Human Sphingosine 1 Phosphate 3 Receptor (EDG-3) CT

Rabbit anti Human Sphingosine 1 Phosphate 3 Receptor (EDG-3) CT

• Background: Endothelial cell differentation gene-3 (EDG3) belongs to a family of G-protein couple receptors whose ligands are lysophospholipids. The ligand for EDG3 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family. They have been implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. The couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG3 is expressed in cardiovascular, leukocyte-containing and other tissues.
• Synonyms: Endothelial Cell differentiation gene 3 C-terminal, Sphingosine-1-Phosphate Receptor-3 (S1P3)
• CAS Number: 9007-83-4
• UniProt: Q99500
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Type: Polyclonal Antibody
• Source: Rabbits were immunized with a synthetic peptide derived from the C terminal of the EDG3 (S1P3) proteins
• Applications: WB, IP
• Field of Research: Signal Transduction
• Purification Method: Ammonium Sulfate Precipitation
• Assay Principle: Antibody can be used for Western blotting (5-10 µg/mL) and Immunoprecipitation (see reference 4). Optimal concentration should be evaluated by serial dilutions. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Goetzl EJ and An, S. Diversity of cellular receptors and functions for the lysophospholipid growth factos lysophosphatidic acid and sphingosine 1-phosphate. FASEB J. 1998: 12, 1589-98._x000D_ 2. An, S., Goetzl, E.J., Lee, H. Signaling mechanisms and molecular characteristics of G protein-coupled receptors for lysophosphatidic acid and sphingosine 1-phosphate. J. Cell Biochem. Suppl. 1998: 30-31, 147-57._x000D_ _x000D_ 3. Singleton PA, Moreno-Vinasco L, Sammani S, Wanderling SL, Moss J, Garcia JG. Attenuation of vascular permeability by methylnaltrexone: role of mOP-R and S1P3 transactivation. Am J Respir Cell Mol Biol. 2007;37(2):222-31._x000D_ _x000D_ 4. Sun X, Singleton PA, Letsiou E, Zhao J, Belvitch P, Sammani S, Chiang ET, Moreno-Vinasco L, Wade MS, Zhou T, Liu B, Parastatidis I, Thomson L, Ischiropoulos H, Natarajan V, Jacobson JR, Machado RF, Dudek SM, Garcia JG. Sphingosine-1-phosphate receptor-3 is a novel biomarker in acute lung injury. Am J Respir Cell Mol Biol. 2012 Nov;47(5):628-36. doi: 10.1165
• Shipping Conditions: Ambient Temperature, freeze upon arrival
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB