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Rabbit anti Human C3a

CAT: 0579-RAHu/C3aSize: 1 mLDry Ice: NoHazardous: No
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CAT#:0579-RAHu/C3aSize:1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against Human plasma a single precipitin line is obtained in the beta-1 region and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of Human serum or plasma. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3a or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.
CAS Number
9007-83-4
UniProt
P01024
Host
Rabbit
Clone
Polyclonal
Source
C3a is a component of complement C3 produced by its activation. It has a molecular weight of 8,900. The protein is isolated and purified from pooled normal Human plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Assay Principle
Precipitation assays. In immunoelectrophoresis in agarose-plates use 2 μl Human plasma or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Form
Delipidated, heat inactivated, lyophilized, stable whole serum; No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of; Normal pooled rabbit serum; No foreign proteins added; Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Storage Conditions
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance of the antiserum Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day

UniProtKB · P01024

Complement C3

CO3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01024
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A7E236
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Disease

Age-related macular degenerationDisease variantHemolytic uremic syndrome

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoproteinThioester bond

Biological process

Complement alternate pathwayComplement pathwayFatty acid metabolismImmunityInflammatory responseInnate immunityLipid metabolism

Cellular component

Secreted

Domain

Signal

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