SingleFlowEx Perforin PE

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

DG9

FC (QC tested)

0.100 mg/mL

PE

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

1 vial of monoclonal antibody reagent.The content (1 mL) is sufficient for min. 100 Tests.

*Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8α (-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011 Nov;134 (3) :326-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/21978002, *Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. J Mol Cell Biol. 2010 Jun;2 (3) :139-51., URL: http://www.ncbi.nlm.nih.gov/pubmed/20348186 , *Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions. PLoS Pathog. 2012;8 (11) :e1002984., URL: http://www.ncbi.nlm.nih.gov/pubmed/23144609 , *Hersperger AR, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry A. 2008 Nov;73 (11) :1050-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/18615597 , *Hameed A, Olsen KJ, Cheng L, Fox WM, Hruban RH, Podack ER: Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992 May;140 (5) :1025-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/1374586 , *Use of this product has not been published., URL: