SingleFlowEx CD59 PE

• Description :

  SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

• Certification :

  RUO (GMP)

• Clone :

  MEM-43

• Applications :

  FC (QC tested)

• Concentration :

  0.040 mg/mL

• Format :

  PE

• Buffer :

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Components :

  1 vial of monoclonal antibody reagent.The content (1 mL) is sufficient for min. 100 Tests.

• References & Citations :

  *IMPORTANT ARTICLE: Robert Sutherland D, Keeney M, Illingworth A: Practical guidelines for the high-sensitivity detection and monitoring of paroxysmal nocturnal hemoglobinuria (PNH) clones by flow cytometry. Cytometry B Clin Cytom. 2012 Apr 12. doi: 10.1002/cyto.b.21023. [Epub ahead of print]. This article recommends PE-conjugated MEM-43 as a good reagent for red blood cell analysis of PNH (Paroxysmal Nocturnal Hemoglobinuria) by flow cytometry., URL: https://www.ncbi.nlm.nih.gov/pubmed/22499484, *Horejsi V, Hilgert I, Kristofova H, Bazil V, Bukovsky A, Kulhankova J: Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) . 1986;32 (1) :12-25. (original description of MEM-43 antigen), URL: https://www.ncbi.nlm.nih.gov/pubmed/2422063?dopt=A, *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989) ., URL: , *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995) ., URL: , *Forsberg UH, Bazil V, Stefanova I, Schroder J: Gene for human CD59 (likely Ly-6 homologue) is located on the short arm of chromosome 11. Immunogenetics. 1989;30 (3) :188-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/2476389?dopt=A, *Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V: Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol. 1989 Feb;26 (2) :153-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/2918859?dopt=A, *Stefanova I, Horejsi V, Ansotegui IJ, Knapp W, Stockinger H: GPI-anchored cell-surface molecules complexed to protein tyrosine kinases. Science. 1991 Nov 15;254 (5034) :1016-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/1719635?dopt=A, *Cinek T, Horejsi V: The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases. J Immunol. 1992 Oct 1;149 (7) :2262-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/1382093?dopt=A, *Bodian DL, Davis SJ, Morgan BP, Rushmere NK: Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59. J Exp Med. 1997 Feb 3;185 (3) :507-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/9053451?dopt=A, *Cebecauer M, Cerny J, Horejsi V: Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells. Biochem Biophys Res Commun. 1998 Feb 24;243 (3) :706-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/9500981?dopt=A, *Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91 (10) :3901-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9573028?itool=, *Omidvar N, Wang EC, Brennan P, Longhi MP, Smith RA, Morgan BP: Expression of glycosylphosphatidylinositol-anchored CD59 on target cells enhances human NK cell-mediated cytotoxicity. J Immunol. 2006 Mar 1;176 (5) :2915-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/16493049?ordin, *Sutherland DR, Richards SJ, Ortiz F, Nayyar R, Benko M, Marinov I, Illingworth A: CD71 improves delineation of PNH type III, PNH type II, and normal immature RBCS in patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom. 2019 Nov 8. doi: 10.1002/cyto.b.21853. , URL: https://www.ncbi.nlm.nih.gov/pubmed/31705743, *Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan;94 (1) :23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353, *Sutherland DR, Richards SJ, Ortiz F, Nayyar R, Benko M, Marinov I, Illingworth A: CD71 improves delineation of PNH type III, PNH type II, and normal immature RBCS in patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom. 2020 Mar;98 (2) :179-192, URL: https://pubmed.ncbi.nlm.nih.gov/31705743/

• Other References :

  *Baalasubramanian S, Harris CL, Donev RM, Mizuno M, Omidvar N, Song WC, Morgan BP: CD59a is the primary regulator of membrane attack complex assembly in the mouse. J Immunol. 2004 Sep 15;173 (6) :3684-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/15356114?ordin, *Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE: CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation. J Immunol. 1994 Sep 15;153 (6) :2444-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/7521361?ordina, *Rooney IA, Davies A, Griffiths D, Williams JD, Davies M, Meri S, Lachmann PJ, Morgan BP: The complement-inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells. Clin Exp Immunol. 1991 Feb;83 (2) :251-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/1704296?ordina, *Meri S, Morgan BP, Davies A, Daniels RH, Olavesen MG, Waldmann H, Lachmann PJ: Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers. Immunology. 1990 Sep;71 (1) :1-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/1698710?ordina

• Storage Conditions :

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.