RegisterLogin

Synthetic Blocker

CAT: 436-EBB300-2Size: 500 mLDry Ice: NoHazardous: No
500 mLSelected
PRICE
AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
Increases the Sensitivity of the Assay. This is done by stabilizing the antigenic and functional regions of the absorbed protein., Reduces Background Noise. Block buffers inhibit nonspecific binding to uncoated regions of the plate and non-specific binding sections of the absorbed proteins. This leads to lower background noise and high specific signal., Increases Efficiency and Extends the Shelf-Life of the Coated Plates. With a longer shelf-life, plates may be prepared in large batches to be used over time. Large batch sizes will increase efficiency and reduce plate-to-plate variability to increase consistency., Conserves Valuable Reagents. By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when the proper blocker is used.
Label
ICT
Type
ELISA & Assay Reagents
Assay Protocol
Once your plate has been coated with an antibody or antigen, aspirate or dump out the coating solution. Then, simply pipette 300-400uL per Well of block buffer and let it incubate (Cell Technology recommends a minimum incubation of 3 hours).
Concentration
1X
Buffer
7.4 at 1X
pH
7.4 at 1X
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Temperature
2-8°C
Shelf Life
Expires two years from date of manufacture
Target Description
ICT’s Synthetic Blocker is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. Because Synthetic Blocker contains inert non-reactive blockers, it reduces the amount of nonspecific binding of enzyme-labeled conjugates to blocked plate surfaces. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives.

Related Products

CatalogName
EBB100-3General-Purpose Blocker
EBB100-1General-Purpose Blocker
EBB100-2General-Purpose Blocker
EBB200-3Nonmammalian-Based Blocker
EBB200-2Nonmammalian-Based Blocker
EBB100-4General-Purpose Blocker
EBB200-4Nonmammalian-Based Blocker
EBB200-1Nonmammalian-Based Blocker
AR-6586-01Endogenous Peroxidase Block
AR-6586-02Endogenous Peroxidase Block

Alternative Products

CatalogName
C04003Blocker
C04004Blocker
C04002Blocker
E52C04004Blocker
E52C04002Blocker
E52C04003Blocker
20960002-2Horse Blocker
20960002-1Horse Blocker
E57V1492Blocker mAb
20960002-2Horse Blocker

Popular Products