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Anti-Peripherin Antibody

CAT: 0588-PERSize: 200 µLDry Ice: NoHazardous: No
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CAT#:0588-PERSize:200 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Human Perpherin is a 53,651 dalton protein (470 amino acids) expressed in all PNS neurons and some CNS neurons, including a subset of cortical neurons, hippocampal neurons, and ventral horn spinal cord motor neurons. At an ultrastructural level, peripherin immunoreactivity is associated with the neurofilament portion of the cytoskeleton, being particularly abundant in the cell body. Peripherin protein has been shown to undergo upregulation during periods of trophic stress in cultured neurons, and mutations of the peripherin gene have been associated with the neurodegenerative disease amyotrophic lateral sclerosis.
Description
Full-length recombinant peripherin was injected into laying hens, and then the IgY fraction was purified from hyperimmunized animals. These antibodies have been validated with human, mouse and rat tissues.
Synonyms
Peripherin, Neurofilament 4
Other Statements
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Gene Name
PRPH NEF4 PRPH1
UniProt
P41219
Host
Chicken
Species Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, and Rat
Immunogen
Recombinant rat peripherin expressed in Escherichia coli
Immunogen Type
Rat
Target
Perpherin
Clonality
Polyclonal
Isotype
IgY
Conjugation
Unconjugated
Applications
IHC, ICC, WB
Field of Research
Cell Markers Neuronal Markers
Assay Type
Primary Antibody
Assay Protocol
Chickens were immunized with full-length recombinant peripherin protein. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks.
Concentration
2 mg/mL
Format
IgY Fraction
Form
IgY Fraction
Buffer
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative
Molecular Weight
60 kDa
Precautions
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Shipping Conditions
Ice Packs
Storage Conditions
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Shelf Life
12 months from receipt of product
IHC Dilution
1:2000-1:5000
WB Dilution
1:2000-1:5000
Pre Treatment
Chickens were immunized with full-length recombinant peripherin protein. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks.
Target Description
Human Perpherin is a 53,651 dalton protein (470 amino acids) expressed in all PNS neurons and some CNS neurons, including a subset of cortical neurons, hippocampal neurons, and ventral horn spinal cord motor neurons. At an ultrastructural level, peripherin immunoreactivity is associated with the neurofilament portion of the cytoskeleton, being particularly abundant in the cell body. Peripherin protein has been shown to undergo upregulation during periods of trophic stress in cultured neurons, and mutations of the peripherin gene have been associated with the neurodegenerative disease amyotrophic lateral sclerosis.
ICC Dilution
1:2000-1:5000
Quality Control
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:10000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Brid
AB_2313558
Uniprot Accession Number
P41219
Physical Properties
Liquid
Isotype
IgY

UniProtKB · P41219

Peripherin

PERI_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P41219
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PRPH
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q8N577
Protein keywords

Coding sequence diversity

Alternative splicing

Disease

Amyotrophic lateral sclerosisDisease variantNeurodegeneration

Cellular component

Cell projectionCytoplasmCytoskeletonIntermediate filament

Domain

Coiled coil

PTM

NitrationPhosphoprotein

Technical term

Proteomics identificationReference proteome
Product Datasheet
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