Anti-Integrin alpha-M (CD11b, ΜΑC-1, OX-42 Antigen) Antibody

• Background:

  We produce our Anti-Integrin Alpha M chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is affinity-purified IgY.

• Description:

  Our anti-Integrin Alpha-M (MAC-1) antibody was purified from eggs collected from chickens injected with a KLH-conjugated peptide corresponding to a sequence shared between the mouse (NP_001076429.1) and human gene products (NP_001139280.1). These antibodies have been validated with human, mouse and rat tissues.

• Synonyms:

  Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD antigen CD11b

• Gene Name:

  ITGAM CD11B CR3A

• UniProt:

  P11215

• Host:

  Chicken

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Human, Mouse

• Immunogen Type:

  Synthetic peptide

• Target:

  Integrin Alpha M

• Clonality:

  Polyclonal

• Isotype:

  IgY

• Conjugation:

  Unconjugated

• Applications:

  IHC, ICC, WB

• Assay Type:

  Primary Antibody

• Concentration:

  100 µg/mL

• Format:

  Liquid

• Form:

  Affinity-Purified IgY

• Buffer:

  Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

• Molecular Weight:

  170 kDa

• Precautions:

  These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

• Shipping Conditions:

  Ice packs

• Storage Conditions:

  Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

• Shelf Life:

  12 months from receipt of product

• IHC Dilution:

  1:2000-1:5000

• WB Dilution:

  1:2000-1:5000

• Pre Treatment:

  Chickens were immunized with a Keyhole Limpet Hemocyanin (KLH)-conjugate of a synthetic peptide corresponding to a region near the center of the extracellular domain of this single-membrane pass gene product). After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column to which the same peptide was attached. Finally, the antibody concentration was adjusted to 100 µg/mL and filter-sterilized.

• Target Description:

  Integrin Alpha M (also known as MAC-1 and CD11b) is a 1142 amino acid protein expressed in various antigen-presenting cells of the immune system, including microglial cells of the central nervous system. This is the same protein that is recognized by the Ox-42 mouse monoclonal antibody.

• ICC Dilution:

  1:2000-1:5000

• Quality Control:

  Antibodies were analyzed by immunohistochemistry (at a concentration of 3 µg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) or HRP-labeled goat anti-chicken IgY (1:2000 dilution, Aves Labs, Cat.# H-1004) as the secondary reagent.

• Brid:

  AB_2313548