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Anti-Olig2

CAT: 0588-OLIG2-0020Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0588-OLIG2-0020Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) is a basic helix-loop-helix (bHLH) transcription factor essential for oligodendrocyte and motoneuron development in the spinal cord (Ono et al., 2008) . OLIG2 is also known to play a key role in mediating the brains response to cortical injury through post-injury gliosis (Chen Y et al., 2008) and is also highly expressed in malignant glioma where it has been implicated in glioma progression.
Description
Our Anti-Olig2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat Olig2, and is affinity-purified IgY. It is great for use in IHC, ICC, WB.
Short Description
Our Anti-Olig2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat Olig2, and is affinity-purified igy. It is great for use in IHC, ICC, WB.
Synonyms
BHLHB1, BHLHE19, PRKCBP2, RACK17
Other Statements
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Gene Name
OLIG2
UniProt
Q13516
Host
Chicken
Species Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, and Rat
Immunogen
N-terminal half of human OLIG2 was used to immunize chickens to avoid cross-reactivity with OLIG3.
Immunogen Type
Human
Target
Olig2
Clonality
Polyclonal
Isotype
IgY
Conjugation
Unconjugated
Applications
IHC, ICC, WB
Field of Research
Cell Markers Glial and Schwann Cell MarkersNeurogenesis and Development Transcription Regulation
Assay Type
Primary Antibody
Assay Protocol
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.
Concentration
0.2 mg/mL
Format
Affinity-Purified IgY
Form
Affinity-Purified IgY
Buffer
Phosphate-buffered saline (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Molecular Weight
35 kDa
Precautions
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Shipping Conditions
Ice Packs
Storage Conditions
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Shelf Life
12 months from receipt of product
IHC Dilution
1:1000
WB Dilution
1:1000
Pre Treatment
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.
Target Description
OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) is a basic helix-loop-helix (bHLH) transcription factor essential for oligodendrocyte and motoneuron development in the spinal cord (Ono et al., 2008). OLIG2 is also known to play a key role in mediating the brains response to cortical injury through post-injury gliosis (Chen Y et al., 2008) and is also highly expressed in malignant glioma where it has been implicated in glioma progression.
ICC Dilution
1:1000
Quality Control
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on lysate from cells expressing Olig2.
Brid
AB_2924438
Uniprot Accession Number
Q13516
Physical Properties
Liquid
Isotype
IgY
Product Datasheet
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