Anti-Olig2

• Background:

  OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) is a basic helix-loop-helix (bHLH) transcription factor essential for oligodendrocyte and motoneuron development in the spinal cord (Ono et al., 2008) . OLIG2 is also known to play a key role in mediating the brains response to cortical injury through post-injury gliosis (Chen Y et al., 2008) and is also highly expressed in malignant glioma where it has been implicated in glioma progression.

• Description:

  Our Anti-Olig2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat Olig2, and is affinity-purified IgY. It is great for use in IHC, ICC, WB.

• Short Description:

  Our Anti-Olig2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat Olig2, and is affinity-purified igy. It is great for use in IHC, ICC, WB.

• Synonyms:

  BHLHB1, BHLHE19, PRKCBP2, RACK17

• Other Statements:

  These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

• Gene Name:

  OLIG2

• UniProt:

  Q13516

• Host:

  Chicken

• Species Reactivity:

  Human, Mouse, Rat

• Reactivity:

  Human, Mouse, and Rat

• Immunogen:

  N-terminal half of human OLIG2 was used to immunize chickens to avoid cross-reactivity with OLIG3.

• Immunogen Type:

  Human

• Target:

  Olig2

• Clonality:

  Polyclonal

• Isotype:

  IgY

• Conjugation:

  Unconjugated

• Applications:

  IHC, ICC, WB

• Field of Research:

  Cell Markers Glial and Schwann Cell MarkersNeurogenesis and Development Transcription Regulation

• Assay Type:

  Primary Antibody

• Assay Protocol:

  Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.

• Concentration:

  0.2 mg/mL

• Format:

  Affinity-Purified IgY

• Form:

  Affinity-Purified IgY

• Buffer:

  Phosphate-buffered saline (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

• Molecular Weight:

  35 kDa

• Precautions:

  These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

• Shipping Conditions:

  Ice Packs

• Storage Conditions:

  Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

• Shelf Life:

  12 months from receipt of product

• IHC Dilution:

  1:1000

• WB Dilution:

  1:1000

• Pre Treatment:

  Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.

• Target Description:

  OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) is a basic helix-loop-helix (bHLH) transcription factor essential for oligodendrocyte and motoneuron development in the spinal cord (Ono et al., 2008). OLIG2 is also known to play a key role in mediating the brains response to cortical injury through post-injury gliosis (Chen Y et al., 2008) and is also highly expressed in malignant glioma where it has been implicated in glioma progression.

• ICC Dilution:

  1:1000

• Quality Control:

  Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on lysate from cells expressing Olig2.

• Brid:

  AB_2924438

• Uniprot Accession Number:

  Q13516

• Physical Properties:

  Liquid

• Isotype:

  IgY