MAP3K4 Polyclonal Antibody

CAT: 0866-RD88697A-01Size: 60 μLDry Ice: NoHazardous: No
CAT#:0866-RD88697A-01Size:60 μL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described.
Description
This is a MAP3K4 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
Synonyms
MAP3K4, MAPKKK4, MEKK 4, MEKK4, MTK1, PRO0412
Gene ID
4216
Swiss Prot
Q9Y6R4
Reactivity
Human, Rat
Immunogen
A synthetic peptide of human MAP3K4
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Polyclonal Antibody
Applications
WB, IHC
Purification Method
Affinity purification
Assay Type
Antibody
Concentration
1mg/mL
Dilution
WB 1:500-1:2000, IHC 1:100-1:200
Buffer
PBS with 0.01% thiomersal,50% glycerol, pH7.3.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
112-130kDa/144kDa/194-231kDa
Observed Molecular Weight
540KDa
Host or Source
Rabbit
Isotype
IgG

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