Anti-Nitrotyrosine (mouse monoclonal) (clone EM-30)

Anti-Nitrotyrosine (mouse monoclonal) (clone EM-30)

• Background: Nitrotyrosinecan be detected in proteins from a variety of tissues, usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: Nitrotyrosine
• Immunogen: NO2-Tyr-CH2-Thyroglobulin
• Clonality: Monoclonal
• Applications: Immunohistochemistry (IHC) paraffin, Iimmunohistochemistry (IHC) frozen sections, Western blot (WB)
• Dilution: (IHC) paraffin: 10 µg/mL; positive tissue: colon cancer epithelium, prostate hyperplasia.(IHC) frozen sections: 10 µg/mL; positive tissue: colon cancer epithelium, prostate hyperplasia.(WB): positive control: NO2-BSA, NO2-HSA.
• Purity: Immunoglobulin purified on Protein A.
• Format: Liquid at 1 mg/mL.
• Storage Conditions: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.