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Anti-mCherry (monoclonal)

CAT: 0451-AS16 3967Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0451-AS16 3967Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
MCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer) . The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals) .
CAS Number
9007-83-4
HS Code
38221900
Host
Mouse
Reactivity
MCherry
Immunogen
Recombinant full length mCherry expressed and purified from E. coli.
Clonality
Monoclonal
Applications
Immunocytochemistry (ICC) (IC), Western blot (WB)
Field of Research
BFP/FITC/GFP/RFP/TRITC/YFP
Dilution
1 : 250-1 : 500 (IC), 1 : 1000-1 : 2000 (WB)
Purity
IgG2a purified from tissue culture supernatant.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Precautions
In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector
References & Citations
Xie et al. (2024) . Proteasome resides in and dismantles plant heat stress granules constitutively. Mol Cell. 2024 Sep 5;84 (17) :3320-3335.e7. doi: 10.1016/j.molcel.2024.07.033.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
MScarlett
Isotype
IgG2a
Product Datasheet
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