Anti-Beta-Actin, (animal), mouse monoclonal

Anti-Beta-Actin, (animal), mouse monoclonal

• Background: Beta-Actinis a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism.  Actin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: Chicken, human, mouse, rabbit, rat
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Synthetic peptide derived from the N-terminal of the human beta-actin
• Clonality: Monoclonal
• Applications: Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
• Dilution: 1: 1000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion
• Purity: IgG2b purified on Protein A n 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
• Format: Lyophilized
• Molecular Weight: 42 kDa
• Precautions: This antibody recognizes both the native and denatured forms of Beta-Actin from a wide range of species
• Storage Conditions: Store at -20°C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°C for several days to weeks