Anti-ACT | Actin (recombinant monoclonal, clone 14H4G8)

Anti-ACT | Actin (recombinant monoclonal, clone 14H4G8)

• Background: Actinis a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
• CAS Number: 9000-83-3
• Host: Mouse
• Reactivity: Arabidopsis thaliana, Medicago sativa, Zea mays
• Not reactive in: Chlamydomonas reinhardtii
• Immunogen: Around 100 amino acids of recombinant actin conserved more than 80% inArabidopsis thaliana: actin-1P0CJ46AT2G37620, actin-2Q96292AT3G18780, actin-3P0CJ47AT3G53750, actin-4P53494AT5G59370, actin-5Q8RYC2At2g42100, actin-7P53492At5g09810, actin-8Q96293AT1G49240, actin-11P53496AT3G12110, actin-12P53497AT3G46520
• Clonality: Monoclonal
• Applications: Immunofluorescence (IF), Western blot (WB)
• Dilution: 1: 500 (IF), 1 : 1000 1: 5000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl, of sterile or deionized water
• Molecular Weight: 41.6 | 45 kDa
• Precautions: This antibody is compatible with a secondary antibody for fluorescence detection:  goat anti-mouse secondary antibody (IRDye 800CW, Li-cor)
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.