Anti-ACT | Actin (polyclonal)

Anti-ACT | Actin (polyclonal)

• Background: Actinis a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Agostis stoloniferacv. ‘Penncross’,Arabidopsis thaliana, Brassicanapus, Cucumis sativus, Cyanthobasis fruticulosa, Cynara cardunculus, Fragaria x ananassa,, Glycine max, Hordeum vulgare, Nicotiana tabacum, Odontarrhena lesbiaca, Petrosimonia nigdeensis, Phaseolus vulgaris, Phaeodactylum tricornutum, Phoenix dactylifera, Picrorhiza kurroa,Salsola grandis, Salsola tragus, Setaria italica, Solanum tuberosum, Triticum aestivum, Vigna unguiculata,Zea mays
• Not reactive in: Chlamydomonas reinhardtii(too high background for this species)
• Immunogen: ca. 100 amino acids of recombinant actin conserved more than 80%  inArabidopsis thaliana:actin-1P0CJ46AT2G37620, actin-2Q96292AT3G18780, actin-3P0CJ47AT3G53750, actin-4P53494AT5G59370, actin-5Q8RYC2At2g42100, actin-7P53492At5g09810, actin-8Q96293AT1G49240, actin-11P53496,AT3G12110,actin-12P53497AT3G46520
• Clonality: Polyclonal
• Applications: immunofluorescence (IF), Western blot (WB)
• Dilution: 1-100 - 1 : 250 (IF), 1 : 3000-1 : 5000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 41.6 | 45 kDa
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• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.