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RuvC (protein, positive control)

CAT: 0451-AS21 4545PSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0451-AS21 4545PSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant.
HS Code
38221900
UniProt
P0A812
Applications
Western blot (WB)
Field of Research
Bacteria
Purity
Contans 50% glycerol, 10 mM Tris-HCl (pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol. Over 95% pure by SDS-PAGE.
Format
Liquid
Molecular Weight
18.7 | 19 kDa
Precautions
RuvC protein is full length, highly purified (over 95%, SDS-PAGE), recombinant without a tag at a concentration of 1 mg/mL (determined by BCA method).  UniProt:P0A814
Additionnal Information
This product can be used in:In vitro functional studies. RuvC cleaves recombination intermediate at Holiday JunctionAs a positive control in Western blot and standard in ELISA.
References & Citations
Shinagawa & Iwasaki (1996) . Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21 (3) :107-11. PMID: 8882584.Iwasaki et al. (1991) Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure. EMBO J. 1991 Dec;10 (13) :4381-9. PMID: 1661673; PMCID: PMC453191.
Storage Conditions
Store at -20°C or -80°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

UniProtKB · P0A812

Holliday junction branch migration complex subunit RuvB

RUVB_ECOLI · Escherichia coli (strain K12)

View on UniProt ↗
Primary accession
P0A812
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ruvB
Protein existence
1: Evidence at protein level
Organism
Escherichia coli (strain K12)
Taxonomy ID
83333
Alternative names
—
EC number
3.6.4.-
Processing
—
Secondary accessions
P08577
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cytoplasm

Biological process

DNA damageDNA recombinationDNA repairSOS response

Molecular function

DNA-bindingHydrolase
Product Datasheet
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