RuvC (protein, positive control)

RuvC (protein, positive control)

• Background: E. coliRuvCprotein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant.
• Applications: Western blot (WB)
• Purity: Contans 50% glycerol, 10 mM Tris-HCl (pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol. Over 95% pure by SDS-PAGE.
• Format: Liquid
• Molecular Weight: 18.7 | 19 kDa
• Precautions: RuvC protein is full length, highly purified (over 95%, SDS-PAGE), recombinant without a tag at a concentration of 1 mg/mL (determined by BCA method).  UniProt:P0A814
• References & Citations: Shinagawa& Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.Iwasakiet al. (1991) Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure. EMBO J. 1991 Dec;10(13):4381-9. PMID: 1661673; PMCID: PMC453191.
• Storage Conditions: Store at -20°C or -80°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.