Anti-Tubulin gamma chain (monoclonal antibodies)

Anti-Tubulin gamma chain (monoclonal antibodies)

• Background: Tubulin gamma chainis essential for the control of microtubular network. Itis found at microtubule organizing centers (MTOC) such as the spindle poles
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: Chlorella vulgaris, Nicotiana tabacum,human, protozoa
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449. UniProt:P23258The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
• Clonality: Monoclonal
• Applications: Immunocytochemistry (ICC), Western blot (WB)
• Dilution: 2-8 µg/mL (ICC) methanol/acetone fixation required, 1-2 µg/mL (WB)
• Purity: Immunoglobulin Protein A purified in PBS. Contains 15 mM sodium azide.
• Format: Liquid
• Molecular Weight: 51 kDa
• Precautions: This antibody recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).The epitope which this antibody is recognizing is conserved inArabidopsis thalianaTubulin gamma-1 chain, UniProt:P38557, Gene ID:At3g61650and Tubulin gamma-2 chain, UniProt:P38558, Gene ID:At5g05620Recommended secondary antibody: goat anti-mouse IgG1AS16 3715
• References & Citations: Romeiro Mottaet al. (2024). The cell cycle controls spindle architecture in Arabidopsis by activating the augmin pathway. Dev Cell. 2024 Aug 23:S1534-5807(24)00484-2. doi: 10.1016/j.devcel.2024.08.001.
• Storage Conditions: Store at 4°C; Do not freeze. Do not exceed exipry date is provided on the tube. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.