Anti-Tubulin beta chain

Anti-Tubulin beta chain

• Background: Tubulin beta (TUB)together with alpha tubulin is making up microtubules.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana(including suspension cells), Chlamydomonas reinhardii, Medicago sativa
• Not reactive in: Allium sativum
• Immunogen: KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including allArabidopsis thalianatubulin beta-2/beta-3 chainP29512, beta-4 chainP24636(At5g44340), beta-5 chainP29513(At1g20010), beta-6 chainP29514(At5g12250), beta-7 chainP29515(At2g29550), beta-8 chainP29516(At5g23860), beta-9 chainP29517(At4g20890).
• Clonality: Polyclonal
• Applications: immunofluorescence (IF), Western blot (WB)
• Dilution: 1 : 1000 (IF), 1 : 500 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 100 µl of sterile water
• Molecular Weight: 49 | 49 kDa (Arabidopsis thaliana)
• Precautions: Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions. More information can be foundhere.
• References & Citations: Gonget al. (2024).HYPK controls stability and catalytic activity of the N-terminal acetyltransferase A in Arabidopsis thaliana. Cell Rep. 2024 Feb 15;43(2):113768.doi: 10.1016/j.celrep.2024.113768.Durianet al. (2019). PROTEIN PHOSPHATASE 2A-B' gamma controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.Wanget al. (2017). The inhibition of protein translation mediated by AtGCN1 is essential for cold tolerance in Arabidopsis thaliana. Plant Cell Environ. 2017 Jan;40(1):56-68. doi: 10.1111/pce.12826.Heinnickelet al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.