Anti-Tubulin alpha chain (polyclonal antibodies)

Anti-Tubulin alpha chain (polyclonal antibodies)

• Background: Tubulin alpha (TUA)together with beta tubulin is making up microtubules.
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardii, Euglena gracilis, Hordeum vulgare, Oryza sativa, Setaria italica, Zea mays
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated peptide derived from available tubulin alpha chain sequences includingArabidopsis thalianatubulin alpha-1-chainP11139(At1g64740), alpha-2/alpha-4 chainB9DGT7(At1g50010), alpha-5 chainB9DHQ0(At5g19780), alpha-6-chainP29511(At4g14960)Peptide used to elict this antibody is not present in tubulin beta.
• Clonality: Polyclonal
• Applications: Immunofluorescence (IF), Western blot (WB)
• Dilution: 1 : 500 (IF), 1 : 1000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 49 | 52 kDa (Arabidopsis thaliana)
• Precautions: Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions. More information can be foundhere.
• References & Citations: Sakurabataet al. (2024). HASTY-mediated miRNA dynamics modulate nitrogen starvation-induced leaf senescence in Arabidopsis. Nat Commun. 2024 Sep 10;15(1):7913. doi: 10.1038/s41467-024-52339-w.Seedpriming with NaCl helps to improve tissue tolerance, potassium retention ability of plants, and protects the photosynthetic ability in two different legumes, chickpea and lentil, under salt stress. Planta. 2023 May 8;257(6):111. doi: 10.1007/s00425-023-04150-y.Zhaoet al (2023) Maize tubulin folding cofactor B is required for cell division and cell growth through modulating microtubule homeostasis.Ye, Zhou, Zhu, et al. (2022) Inhibition of shoot-expressed NRT1.1 improves reutilization of apoplastic iron under iron-deficient conditions. Plant J. 2022;112(2):549-564. doi:10.1111/tpj.15967Grafet al. (2024). D6PK plasma membrane polarity requires a repeated CXX (X) P motif and PDK1-dependent phosphorylation. Nat Plants. 2024 Feb;10(2):300-314.Kafriet al. (2023). Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25.doi: 10.1016/j.cell.2023.11.007.Kumaret al. (2022). Proteomic dissection of rice cytoskeleton reveals the dominance of microtubule and microfilament proteins, and novel components in the cytoskeleton-bound polysome, Plant Physiology and Biochemistry, Volume 170,2022,Pages 75-86,ISSN 0981-9428, https://doi.org/10.1016/j.plaphy.2021.11.037.Guet al. (2021) A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green Alga Chlamydomonas reinhardtiiSakurabaat al. (2020). Multilayered regulation of membrane-bound ONAC054 is essential for abscisic acid-induced leaf senescence in rice. Plant Cell. 2020 Jan 6. pii: tpc.00569.2019. doi: 10.1105/tpc.19.00569.Roustanet al. (2020). Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s. Sci Rep. 2020 Feb 5;10(1):1864. doi: 10.1038/s41598-020-58740-x.Roustanet al. (2020). Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s. Sci Rep. 2020 Feb 5;10(1):1864. doi: 10.1038/s41598-020-58740-x.Upadhyayaand Jagadeeshwar Rao (2019). Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii. Plant Direct Volume 3, Issue 11.Liet al. (2019). A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nat Genet. 2019 Apr;51(4):627-635. doi: 10.1038/s41588-019-0370-6.Panet al. (2018). Comparative proteomic investigation of drought responses in foxtail millet. BMC Plant Biol. 2018 Nov 29;18(1):315. doi: 10.1186/s12870-018-1533-9.Nasiret al. (2018). Identification of a flagellar protein implicated in the gravitaxis in the flagellate Euglena gracilis. Sci Rep. 2018 May 15;8(1):7605. doi: 10.1038/s41598-018-26046-8.Kwonet al. (2018). AtCAP2 is crucial for lytic vacuole biogenesis during germination by positively regulating vacuolar protein trafficking. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):E1675-E1683. doi: 10.1073/pnas.1717204115.Hoet al. (2017). A calcineurin B-like protein participates in low oxygen signalling in rice. CSIRO PUBLISHING Functional Plant Biology.Weiet al. (2017). Light Intensity is Important for Hydrogen Production in NaHSO3-Treated Chlamydomonas reinhardtii. Plant Cell Physiol. 2017 Mar 1;58(3):451-457. doi: 10.1093/pcp/pcw216.Nasir(2016). Analysis of signal transduction chains of gravity and light sensing in Euglena gracilis. Doctoral Thesis, urn:nbn:de:bvb:29-opus4-79185.Armbrusteret al. (2014). Ion antiport accelerates photosynthetic acclimation in fluctuating light environments. Nat Commun. 2014 Nov 13;5:5439. doi: 10.1038/ncomms6439Juszczaket al. (2012). Natural genetic variation in the expression regulation of the chloroplast antioxidant system among Arabidopsis thaliana accessions. Physiol. Plant.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.