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Anti-EIN3 | Ethylene insensitive 3

CAT: 0451-AS19 4273Size: 50 µLDry Ice: NoHazardous: No
CAT#:0451-AS19 4273Size:50 µL
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Background
EIN3 (Ethylene insensitive 3)acts as a positive regulator in the ethylene response pathway and is required for ethylene responsiveness in adult plant tissues. Activated by phosphorylation by MPK3 and MPK6 (PubMed:18273012). Down-regulated by KIN10 that controls its protein stability under a phosphorylation-dependent manner.Alternative names: Protein ethylene insensitive 3.
CAS Number
9007-83-4
Host
Rabbit
Reactivity
Arabidopsis thaliana
Not reactive in
Medicago truncatula
Immunogen
Recombinant EIN3 ofArabidopsis thaliana, UniProt:O24606, TAIR:At3g20770
Clonality
Polyclonal
Applications
Western blot (WB)
Dilution
1 : 1000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl, of sterile water
Molecular Weight
71.4 | 78 kDa
Precautions
Western blot condtions for anti-EIN3 antibodies are described inSmalle et al. 2002.Recommended membrane for blocking: nitrocellulose, blocking reagent: 10 % non-fat milk, antibody incubation buffer: PBS-T.
References & Citations
Parket al. (2023) Ethylene-triggered subcellular trafficking of CTR1 enhances the response to ethylene gas. Nat Commun. 2023;14(1):365. Published 2023 Jan 23. doi:10.1038/s41467-023-35975-6Gagneet al. (2004). Arabidopsis EIN3-binding F-box 1 and 2 form ubiquitin-protein ligases that repress ethylene action and promote growth by directing EIN3 degradation. Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6803-8.
Storage Conditions
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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